Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: PLOS ONE

Article Title: Evaluation of decellularization process for developing osteogenic bovine cancellous bone scaffolds in-vitro

doi: 10.1371/journal.pone.0283922

Figure Lengend Snippet: Bar charts indicate comparison between DCC scaffold vs DCC scaffold + cells and DMB scaffolds vs DMB scaffold + cells by direct method (a). Bar charts indicate comparison between DMB conditioned media (CM) and DCC conditioned media (CM) vs complete culture media (Control) by indirect method (b). *p<0.05 is indicated. n = 4; CM: conditioned media. (c). HOB cells seeded on DMB and DCC scaffolds showing optimum cell attachment by DAPI staining. (i & iii) DMB scaffolds and (ii & iv) DCC scaffolds; images were captured by fluorescence and confocal microscopy, respectively. Scanning electron micrograph of HOB cells seeded onto DMB (d) and DCC (e) scaffolds. The osteoblast shows a healthy morphology residing on the ECM with evidence of filopodia spread (red arrows).

Article Snippet: Human osteoblast cells (HOB) used for biocompatibility evaluation were obtained from Addexbio (Cat no: P0004010; USA).

Techniques: Comparison, Control, Cell Attachment Assay, Staining, Fluorescence, Confocal Microscopy

Journal: PLOS ONE

Article Title: Evaluation of decellularization process for developing osteogenic bovine cancellous bone scaffolds in-vitro

doi: 10.1371/journal.pone.0283922

Figure Lengend Snippet: Photomicrographs of HOB cells stained with Alizarin Red S. HOB cells showed increased mineralized nodules in osteogenic medium (OS + ) compared to cells without supplements (OS - ). More significant mineralization was seen by day 14 (a). Quantification of alizarin Red S stained mineralized nodules in human osteoblast cells seeded on DMB and DCC scaffolds grown in the presence or absence of osteogenic induction media at days 7 and 14 in comparison to cells alone is shown; p<0.001 is indicated). The absorbance of the extracted Alizarin dye was measured at 405 nm. Data presented as mean ± SEM (n = 6) (b). Quantification of Alizarin Red S staining in osteoblast cell-seeded DMB and DCC scaffolds on day 14 in the presence and absence of osteogenic supplements. Data presented as mean ± SEM (n = 4) (c); p<0.001 is indicated.

Article Snippet: Human osteoblast cells (HOB) used for biocompatibility evaluation were obtained from Addexbio (Cat no: P0004010; USA).

Techniques: Staining, Comparison